Search results for "pathology [Gray Matter]"
showing 10 items of 1707 documents
Deglycosylated bleomycin triggers apoptosis in laryngeal carcinoma cells in a caspase and reactive oxygen species independent manner
2008
Background: Bleomycin-A2, a member of a family of glycopeptide antibiotics, has potent antitumor activity against a range of lymphomas, head and neck cancer and germ cell tumors. However, little is known about the biologic activity of the carbohydrate moiety in Bleomycin-A2-induced cytotoxicity. Methods: We compared the capacity of Bleomycin-A2 and its deglycosylated form (deglycoBleomycin-A2) to induce cell death. Apoptosis was analyzed by cell nuclear staining with Hoechst 33342 dye and DNA fragmentation. The signal transduction pathway was measured through Western blotting and production of reactive oxygen species (ROS). Results: When tested on HEp-2 laryngeal carcinoma cells, Bleomyc…
Peroxisomes and Hepatotoxicity
1995
Peroxisomes are ubiquitous organelles of eukaryotic cells and are present in significant amounts in hepatic liver cells. Peroxisomal enzymes contribute to several metabolic pathways including fatty acid, purine and amino acid catabolism or bile acid synthesis. The peroxisomal oxidative reactions produce hydrogen peroxide, mostly degraded by catalase which prevents oxidative stress. Moreover, peroxisomes are involved in arylderivative drug detoxification through its epoxide hydrolase activity.
The chemistry of death--Adipocere degradation in modern graveyards.
2015
The formation of adipocere slows further decomposition and preserves corpses for decades or even centuries. This resistance to degradation is a serious problem, especially with regard to the reuse of graves after regular resting times. We present results from an exhumation series in modern graveyards where coffins from water-saturated earth graves contained adipocere embedded in black humic material after resting times of about 30 years. Based on the assumption that this humic material resulted from in situ degradation of adipocere, its presence contradicts the commonly held opinion that adipocere decomposition only occurs under aerobic conditions. To test our hypothesis, we collected black…
Biochemical identification and tissue-specific expression patterns of keratins in the zebrafish Danio rerio
1998
We have identified a number of type I and type II keratins in the zebrafish Danio rerio by two-dimensional polyacrylamide gel electrophoresis, complementary keratin blot-binding assay and immunoblotting. These keratins range from 56 kDa to 46 kDa in molecular mass and from pH 6.6 to pH 5.2 in isoelectric point. Type II zebrafish keratins exhibit significantly higher molecular masses (56-52 kDa) compared with the type I keratins (50-48 kDa), but the isoelectric points show no significant difference between the two keratin subclasses (type II: pH 6.0-5.5; type I: pH 6.1-5.2). According to their occurrence in various zebrafish tissues, the identified keratins can be classified into "E" (epider…
Nachweis von alpha1-Fetoprotein an Blutspuren mit Hilfe der Celluloseacetatfolien-(CAF)-Elektrophorese
1977
Die von Patzelt, Geserick und Lignitz fur Agargelelektrophorese zum Nachweis von alpha1-Fetoprotein beschriebene Technik konnte mit Erfolg auf das Celluloseacetatfolien-(CAF)-System ubertragen werden. Die Unterscheidbarkeit von Neugeborenen- oder Fetalblut und Erwachsenenblut wurde an 219 Neugeborenenbluten gepruft, die auf verschiedenen Spurentragern (Glas, Holz, Filterpapier und Leinen) getrocknet worden waren und gelang ausnahmslos nach Lagerung bei Zimmertemperatur bis zu einem Spurenalter von 8 Monaten. Die Spurentrager beeinflusten die Darstellung nicht.
Einflu� von Medikamenten auf den Acetaldehydspiegel im Blut nach Alkoholzufuhr (enzymatische Bestimmung des Acetaldehyd)
1957
Mit einer enzymatischen und damit spezifischen Bestimmung des Acetaldehyd in Korperflussigkeiten konnte folgendes festgestellt werden. 1. Im Blutserum von Ratten konnte in der Mehrzahl der Falle keine Acetaldehydkonzentration bestimmt werden, im Einzelfall jedoch bis zu 0,09 mg- %. Die bisher in der Literatur angegebenen Normwerte hinsichtlich des Acetaldehydgehaltes, die aber mit relativ unspezifischen Methoden bestimmt worden waren, liesen sich an Ratten nicht bestatigen. 2. Die bisher mitgeteilten Konzentrationen an Acetaldehyd nach Verabreichung einer Sattigungsdosis von Antabus und Alkohol liegen uber 2 mg- %. Auch bei Uberprufung dieser Versuchsanordnung konnte niemals die Hohe dieser…
Changes in the DNA profile caused by toxic substances?
1997
Hating Adolescents Test (HAT): a preliminary development of a measure to assess hating among adolescents
2020
The phenomenon of haters is becoming common among adolescents. The aims of the present research were to evaluate the preliminary psychometric properties of the Hating Adolescents Test (HAT), an ad hoc questionnaire created to evaluate online and offline hate (Study 1), and possible risk factors connected with hate (Study 2). Participants (202 female and 200 male) of this study completed the HAT, the How I Think Questionnaire, the Buss–Perry Aggression Questionnaire, and the Penn State Worry Questionnaire. Descriptive statistics were calculated, and exploratory factor analysis and confirmatory factor analysis were applied. Preliminary data suggest how males reported higher level of hate than…
Comparing deep‐end confinement in England & Wales and Norway
2022
Extreme forms of custody represent the boundary points of state power. The configuration of the most restrictive corners of prison systems, and what goes on within them, is highly instructive in exposing the objectives, limits, and implications of state coercion at its most severe. Based on data collected in England & Wales and Norway, this article has two main aims. The first is to explore the degree to which “deep-end” confinement differs between jurisdictions with different penal philosophies. The second is to understand how the most extreme form of confinement in each jurisdiction differs from the more typical carceral experiences within each system and its overall penal ethos. Empirica…
Fabry Disease With Concomitant Lewy Body Disease
2019
AbstractAlthough Gaucher disease can be accompanied by Lewy pathology (LP) and extrapyramidal symptoms, it is unknown if LP exists in Fabry disease (FD), another progressive multisystem lysosomal storage disorder. We aimed to elucidate the distribution patterns of FD-related inclusions and LP in the brain of a 58-year-old cognitively unimpaired male FD patient suffering from predominant hypokinesia. Immunohistochemistry (CD77, α-synuclein, collagen IV) and neuropathological staging were performed on 100-µm sections. Tissue from the enteric or peripheral nervous system was unavailable. As controls, a second cognitively unimpaired 50-year-old male FD patient without LP or motor symptoms and 3…